Reprod Fertil Dev. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Get the latest research from NIH: https://www.nih.gov/coronavirus. Performed the experiments: ZB XG QZ JL WH HQY JC.
Finally, phylogenetic analysis showed that the intestinal content, feces and soil samples all contained the same dominant group of bacteria. (2015) The Effects of GH Transgenic Goats on the Microflora of the Intestine, Feces and Surrounding Soil.
Feces were taken from GH transgenic and non-transgenic goats, and each sample was taken when it just been defecated. Click through the PLOS taxonomy to find articles in your field.
State of the art in the production of transgenic goats.
1997) and the feasibility of the large-scale production for the industrial application has been confirmed (Baldassarre et al. Increased efficiency of transgenic livestock production.
Pronuclear embryo yield in Canindé and Saanen goats for DNA microinjection. Is the Subject Area "Gastrointestinal tract" applicable to this article? Yes Khan IT, Bule M, Ullah R, Nadeem M, Asif S, Niaz K. Vet World. Transgenic goats produce milk with an anticoagulant that is then extracted for use in the drug ATryn. To date, horizontal gene transfer between GE animals and bacteria has not been reported. COVID-19 is an emerging, rapidly evolving situation.
-, Theriogenology. https://doi.org/10.1371/journal.pone.0139822, Editor: Xiuchun (Cindy) Tian, University of Connecticut, UNITED STATES, Received: April 28, 2015; Accepted: September 16, 2015; Published: October 7, 2015, Copyright: © 2015 Bao et al.
-, Nat Biotechnol. The samples are described in Table 2. The gels were run at 150 V for 420 min and then silver stained. No, Is the Subject Area "Polymerase chain reaction" applicable to this article? College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China, Affiliation
Keywords: Affiliation HHS
2001 Dec;10(6):577-82 The intestinal flora of mature livestock should be stable. The method to produce transgenic goats using this method can be summarized in the following steps: obtaining pronuclear embryos (in vivo or in vitro), embryo microinjection with a DNA construct, transfer of microinjected embryos into recipient goats, and early pregnancy diagnosis by ultrasound. Epub 2019 Jan 5. https://doi.org/10.1371/journal.pone.0139822.g002.
https://doi.org/10.1371/journal.pone.0139822.s003, https://doi.org/10.1371/journal.pone.0139822.s004. GE, Ethics statement . (C) PCR result of the neoR gene detection. Freitas VJ, Serova IA, Andreeva LE, Júnior ES, Teixeira DI, Cordeiro MF, Rondina D, Paula NR, Arruda IJ, Verde JB, Dvoriantchikov G, Serov O. Maga EA, Sargent RG, Zeng H, Pati S, Zarling DA, Oppenheim SM, Collette NM, Moyer AL, Conrad-Brink JS, Rowe JD, BonDurant RH, Anderson GB, Murray JD.
Competing interests: The authors have declared that no competing interests exist. PCR products were subjected to DGGE analysis with the Dcode Universal Mutation Detection System (Bio-Rad, Hercules, USA).
Discover a faster, simpler path to publishing in a high-quality journal. The DGGE profiles for the total microbial DNAs extracted from samples are shown. No, Is the Subject Area "Genetically modified animals" applicable to this article?
GH transgenic goats were confirmed by PCR analysis and verified the transgenic copy number and integration sites [13, 14].
PCR amplification was carried out in 50 μl reaction volumes, composed of 5 μl of 10 × PCR buffer, 4 μl of dNTP mixture, 1 μl of each primer (20 μM), 0.25 μl rTaq polymerase (5 U/μl), 2.5 ng of temple DNA and ddH2O to 50 μl.
We also demonstrated that the foreign GH gene and neoR gene did not change the microbial flora in the goat intestine or the surrounding soil. These products were sequenced with an ABI Prism Big Dye Terminator Cycle Sequencing Ready Kit (Applied Biosystems, USA) with a pair of universal primers for the pMD19-T vector: M13F (-40) GTTTCCCAGTCACGAC and M13R (-26) CAGGAAACAGCTATGAC. Analyzed the data: ZB XG QZ ZB XG.
Production of transgenic founder goats involves introducing and stably integrating an engineered piece of DNA into the genome of the animal. In conclusion, we did not find any evidence of horizontal gene transfer from the GH transgenic goats to the gut floral of other goats or soil microorganisms. All the fresh samples are stored in -70°C before analysis. Baldassarre H, Wang B, Keefer CL, Lazaris A, Karatzas CN. The 16S rDNA genes were amplified with an initial denaturation of DNA at 94°C for 10 min, followed by 30 cycles of 60 s of denaturation at 94°C, 60 s annealing at 55°C, 90 s of elongation at 72°C, and a final elongation for 10 min at the same temperature. Transgenic Goats Original paragraph The animals look and act just like regular goats: Workers milk them like they would at any dairy, and the goats raid their handlers’ pockets for stuff to chew on—a risk you face around any kid, doe, or billy goat. The numbers in the figures indicate the DGGE bands selected for cloning and sequencing. The microbial community of the gastrointestinal tract is closely associated with the host metabolism and has a complex and sensitive construction [11]. PC: positive control with pcGH vector over-expression. Each prominent DGGE band was recovered from the gels, cloned and sequenced.
Somatic cell nuclear transfer; neoR, PLoS ONE 10(10): It is therefore necessary to detect bacterial changes in the feces of GE livestock in any complete environmental assessment. Yes She is the first Latin American transgenic goat produced through the technique of cloning using genetically modified cells. |
No, Is the Subject Area "Goats" applicable to this article?
Here, we focused on the effects of the GH transgenic goat on the microflora of the intestine, feces and surrounding soil. here. 2004;16(4):465-70. doi: 10.10371/RD04028. Citation: Bao Z, Gao X, Zhang Q, Lin J, Hu W, Yu H, et al.
Reprod Domest Anim.
https://doi.org/10.1371/journal.pone.0139822.g004. But scientists have added an extra protein or two to their already nutrient-rich milk. https://doi.org/10.1371/journal.pone.0139822.s001. The results showed that the extracted DNA from all samples contained the bacterial gene. However, the safety evaluation of GE animals and food products should be considered seriously, especially with regard to potential effects on microflora through possible horizontal gene transfer.
The microbial community of the gut can be affected by many factors, including animal health [17], age [18] and foraging patterns [19]. -, Biotechnology (N Y).
https://doi.org/10.1371/journal.pone.0139822.s002. This work was supported by grants from the National Animal Transgenic Breeding Grant Project (No.
Substantially contributed to the concept and design of the work: QY QHY. Soil samples were taken from 0 m to 150 m from the GH transgenic goats’ pen with 15m in width and 30m in length. Moura RR, Lopes-Junior ES, Teixeira DI, Serova IA, Andreeva LE, Melo LM, Freitas VJ. Her name comes from a protein she carries in her milk, glucocerebrosidase, which works to process glucocerebrosides, a type of cell lipid. NC: negative control with ddH2O. To date, many GE animals have been produced, including fish [1], mice [1], rabbits [2], sheep [2], pigs [2, 3], cows [4], and goats [5]. Contributed reagents/materials/analysis tools: ZB XG QZ HQY JC.
The PCR amplifications were carried out in 20 μl reaction volumes comprised of 10 μl of 2 × Taq Master Mix, 1 μl of each primer (10 μM), 0.2 μg of temple DNA and added ddH2O to 20 μl. All animal care and use procedures were conducted in strict accordance with the Animal Research Committee guidelines of the College of Veterinary Medicine at Nanjing Agricultural University. Epub 2017 Dec 16. here. We slaughtered the goats and cut the intestine lengthwise to collect the intestinal contents from jejunum and cecum. Epub 2016 Apr 21.
The samples details were performed in Table 2.
2016 Jul 1;86(1):160-9. doi: 10.1016/j.theriogenology.2016.04.028.
Birth of normal kids after microinjection of pronuclear embryos in a transgenic goat (Capra hircus) production program in Brazil.
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