So let’s see what is it in actually. Application of Transgenic animals:-Biotechnology:-In biotechnology, the transgenic animals can be used in the research purposes. These animals are prepared by modifying sperms from male or eggs from female or it may be modifying new formed embryo. The most commonly used techniques for producing transgenic mice involves either the pronuclear injection of transgenes into fertilized oocytes or embryonic stem cell-mediated gene targeting. The technology for producing transgenic animals exists for a variety of vertebrate and invertebrate species. The most common method for producing transgenic animals is gene transfer by DNA microinjection, which involves the following steps: DNA containing the desired transgene is identified and cloned (copied tens of thousands of times in bacteria) before insertion into the animal host. Two methods of producing transgenic mice are widely used: transforming embryonic stem cells (ES cells) growing in tissue culture with the desired DNA; injecting the desired gene into the pronucleus of a fertilized mouse egg; Fig.11.4.1 Methods to produce Transgenic mice US5567607A US08/424,221 US42422195A US5567607A US 5567607 A US5567607 A US 5567607A US 42422195 A US42422195 A US 42422195A US 5567607 A US5567607 A US 5567607A Authority US United States Prior art keywords dna method according electric field pulses electrode Prior art date … Transgenic animals are nothing but the genetically modified (GM) animals or organism. The mouse is the most utilized organism for research in neurodegenerative diseases. Such therapeutic proteins are currently produced in mammalian cell-based reactors, but this production process is expensive. 2010 Mar;214(2-3):91-109. doi: 10.1007/s00429-009-0230-8. The host animals (cows, pigs, or sheep) are induced to superovulate and their eggs are collected. Epub 2009 Nov 25. Using the techniques of recombinant DNA technology or genetic engineering, the health of animals can be enhanced by making changes in their genes. … Animal transgenesis: an overview Brain Struct Funct. For example, insect-resistant transgenic plants have been engineered. The mouse is the most utilized organism for research in neurodegenerative diseases. It also includes test tube baby, rDNA technology. The technology for producing transgenic animals exists for a variety of vertebrate and invertebrate species. PCT/DK93/00024 teaches methods for producing human kappa-casein in the milk of transgenic animals. 8) International Patent Application No. Transgenic techniques have been used for a number of goals: to determine an unknown gene's function; to analyze the malfunction of a mutated gene; to model human disease; and to provide better agricultural and pharmaceutical products by making transgenic plants and animals. Transgenic farm animals are also being explored as a means to produce large quantities of complex human proteins for the treatment of human disease. ... gene therapy and other genetic engineering methods. Method of producing transgenic animals Download PDF Info Publication number US5567607A. This published patent application also teaches methods of producing and using the altered milk obtained from these transgenic animals, and a method for obtaining only the primary gene product in the milk of the transgenic mammal.
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