Reviews, Request Copyright © 2020 Elsevier B.V. or its licensors or contributors. These authors contributed equally to this work. This in vivo model confirms bioavailability of vector through amniotic fluid with some cross-infection of adjacent fetuses.

Alba was the name of a genetically modified "glowing" rabbit created as an artistic work by contemporary artist Eduardo Kac, produced in collaboration with French geneticist Louis-Marie Houdebine.. Houdebine used the GFP gene found in the jellyfish, Aequorea victoria, that fluoresces green when exposed to blue light. To our knowledge, however, the ability to optically image GFP in … https://doi.org/10.1016/j.jpedsurg.2005.08.047. These results demonstrate the potential application of optical imaging of GFP to monitor gene transfer and track gene ex-pression. Microinjection of 8–16 cell stage embryos resulted extraembryonic gene expression. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. By continuing you agree to the use of cookies. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Lentiviral vector–mediated, in vivo gene transfer to the tracheobronchial tree in fetal rabbits. Fetal gene replacement is a novel, potential therapy for antenatally diagnosed monogenic disorders. Lentiviral vector–mediated GFP gene transfer to fetal rabbit tracheobronchial epithelium occurs within 4 days of transfection by both amniotic injection or direct fetal tracheal injection. In one prep, GFP DNA was identified in maternal lung. Lentiviral vector–mediated GFP gene transfer to fetal rabbit tracheobronchial epithelium occurs within 4 days of transfection by both amniotic injection or direct fetal tracheal injection. A strikingly high degree of mosaic GFP expression was detected in some parts of the yolk sac, which is a hypoblast-derived tissue.

The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species. The purpose of this study was to evaluate in vivo techniques of lentiviral (LV) vector–mediated gene transfer to the tracheobronchial tree in a rabbit model of fetal gene therapy. As rabbits are an ideal model for functional studies in the placenta, our method would open new possibilities in rabbit biotechnology and placentation studies. Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). Generation of rabbits with placenta-speci fi c gene transfer A lentiviral construct with GFP as a reporter gene under the control of the EF1 promoter was microinjected into the perivitelline space of Strong but mosaic reporter gene expression in the placenta and yolk sac. distribution of gene transfer in the eye (11), tumors (12), and the airway (13). Some of this luminescent energy is transferred to the GFP, shifting the overall color towards green. Via triple plasmid transfection, vesicular stomatitis virus-G–pseudotyped LV vector containing green fluorescent protein (GFP) reporter gene under the control of a cytomegalovirus promoter was constructed. In this report we show that genetic modification can be produced in the extraembryonic tissues of rabbits by lentiviral gene constructs. Whereas expression of the reporter gene construct was detected in placentas and yolk sacs, fetuses never expressed the transgene. Copyright © 2020 OriGene Technologies, Inc. All Rights Reserved. Fetal and maternal tissues were analyzed. The expression pattern displayed some mosaicism. Placenta-specific Gene Manipulation in Rabbits with lentiviral vectors. Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation. Presented at the 38th Annual Meeting of the Pacific Association of Pediatric Surgeons, May 22-26, 2005, Vancouver, Canada. Vector access to fetal tissues appears to be by both luminal and hematogenous routes. Lentiviral gene constructs can be efficiently and specifically delivered to trophoblast cell lineages in rodents. Transgenic rabbits expressing GFP as a reporter gene using lentiviral technology have been already reported. ,Ling YH, Wong CC, Li KW, Chan KM, Boukamp P, Liu WK, HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. ,Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V, Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates, Customer Copyright © 2020 Elsevier B.V. or its licensors or contributors. By continuing you agree to the use of cookies. In vivo genetic manipulation of trophoblast cell lines enables functional and developmental studies in the placenta. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. Mosaic expression was found in all founders, and the germline transmission of the transgene was limited Hiripi et al., 2010). SIV-derived lentiviral constructs were used to efficiently produce transgenic rabbit founders. The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal.

Transplacental gene transfer from fetus to mother may occur in this model. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Placenta-specific gene manipulation in rabbits. In vivo gene transfer of 5 × 106 LV particles to fetuses of time-mated NZW rabbits (term = 31 days) was attempted using 2 techniques: (1) direct amniotic injection (gestation = 24-26 days) and (2) direct tracheal injection (gestation = 26 days). We use cookies to help provide and enhance our service and tailor content and ads. Injected fetuses and saline-injected littermate controls were delivered and killed on gestational day 30. However, its utility as a tool for molecular biologists did not begin to be realized until 1992 when Douglas Prasher reported the cloning and nucleotide sequence of wtGFP in Gene. Copyright © 2005 Elsevier Inc. All rights reserved. The rabbit is a common animal model that has been employed in studies on various human disorders and the generation of genetically modified rabbit lines is highly desirable. Published by Elsevier B.V. https://doi.org/10.1016/j.jbiotec.2017.07.037.

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